This age saw the Greeks move towards larger cities and a reduction in the importance of the city-state. These larger cities were parts of the still larger Kingdoms of the Diadochi. Greeks, however, remained aware of their past, chiefly through the study of the works of Homer and the classical authors. An important factor in maintaining Greek identity was contact with barbarian (non-Greek) peoples, which was deepened in the new cosmopolitan environment of the multi-ethnic Hellenistic kingdoms. This led to a strong desire among Greeks to organize the transmission of the Hellenic paideia to the next generation. Greek science, technology and mathematics are generally considered to have reached their peak during the Hellenistic period.
What academia was heightened during this time of Hellenism ?
Greek science, technology and mathematics are generally considered to have reached their peak during the Hellenistic period.

This time they succeeded, and on 31 December 1600, the Queen granted a Royal Charter to "George, Earl of Cumberland, and 215 Knights, Aldermen, and Burgesses" under the name, Governor and Company of Merchants of London trading with the East Indies. For a period of fifteen years the charter awarded the newly formed company a monopoly on trade with all countries east of the Cape of Good Hope and west of the Straits of Magellan. Sir James Lancaster commanded the first East India Company voyage in 1601 and returned in 1603. and in March 1604 Sir Henry Middleton commanded the second voyage. General William Keeling, a captain during the second voyage, led the third voyage from 1607 to 1610.
What did this charter give them?
a monopoly on trade with all countries east of the Cape of Good Hope and west of the Straits of Magellan

The simplest known circadian clock is that of the prokaryotic cyanobacteria. Recent research has demonstrated that the circadian clock of Synechococcus elongatus can be reconstituted in vitro with just the three proteins (KaiA, KaiB, KaiC) of their central oscillator. This clock has been shown to sustain a 22-hour rhythm over several days upon the addition of ATP. Previous explanations of the prokaryotic circadian timekeeper were dependent upon a DNA transcription/translation feedback mechanism.[citation needed]
How long a rhythm were researchers able to sustain in the in vitro experiment?
22-hour