Problem: Symbiosis:

Adaptation of the endosymbiont to the host's lifestyle leads to many changes in the endosymbiont–the foremost being drastic reduction in its genome size. This is due to many genes being lost during the process of metabolism, and DNA repair and recombination. While important genes participating in the DNA to RNA transcription, protein translation and DNA/RNA replication are retained. That is, a decrease in genome size is due to loss of protein coding genes and not due to lessening of inter-genic regions or open reading frame (ORF) size. Thus, species that are naturally evolving and contain reduced sizes of genes can be accounted for an increased number of noticeable differences between them, thereby leading to changes in their evolutionary rates. As the endosymbiotic bacteria related with these insects are passed on to the offspring strictly via vertical genetic transmission, intracellular bacteria goes through many hurdles during the process, resulting in the decrease in effective population sizes when compared to the free living bacteria. This incapability of the endosymbiotic bacteria to reinstate its wild type phenotype via a recombination process is called as Muller's ratchet phenomenon. Muller's ratchet phenomenon together with less effective population sizes has led to an accretion of deleterious mutations in the non-essential genes of the intracellular bacteria. This could have been due to lack of selection mechanisms prevailing in the rich environment of the host.

What happens when the DNA/RNA replications are retained?
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A: unanswerable


Problem: In 1972, Walter Fiers and his team at the University of Ghent were the first to determine the sequence of a gene: the gene for Bacteriophage MS2 coat protein. The subsequent development of chain-termination DNA sequencing in 1977 by Frederick Sanger improved the efficiency of sequencing and turned it into a routine laboratory tool. An automated version of the Sanger method was used in early phases of the Human Genome Project.
What was the first gene to be sequenced?
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Answer: the gene for Bacteriophage MS2 coat protein


Q: What is a question about this article? If the question is unanswerable, say "unanswerable".
Middle Persian (Pahlavi) was the official language under the Sasanian dynasty in Iran. It was in use from the 3rd century CE until the beginning of the 10th century. The script used for Middle Persian in this era underwent significant maturity. Middle Persian, Parthian and Sogdian were also used as literary languages by the Manichaeans, whose texts also survive in various non-Iranian languages, from Latin to Chinese. Manichaean texts were written in a script closely akin to the Syriac script.
What are three spoken languages use by the Manchaeans?
A: unanswerable


Context and question: The era of planning in Himachal Pradesh started 1948 along with the rest of India. The first five-year plan allocated ₹ 52.7 million to Himachal. More than 50% of this expenditure was incurred on road construction since it was felt that without proper transport facilities, the process of planning and development could not be carried to the people, who mostly lived an isolated existence in far away areas. Himachal now ranks fourth in respect of per capita income among the states of the Indian Union.
Where does India rank regarding per capita income?
Answer: unanswerable


Question: In many ways, the Paleocene continued processes that had begun during the late Cretaceous Period. During the Paleocene, the continents continued to drift toward their present positions. Supercontinent Laurasia had not yet separated into three continents. Europe and Greenland were still connected. North America and Asia were still intermittently joined by a land bridge, while Greenland and North America were beginning to separate. The Laramide orogeny of the late Cretaceous continued to uplift the Rocky Mountains in the American west, which ended in the succeeding epoch. South and North America remained separated by equatorial seas (they joined during the Neogene); the components of the former southern supercontinent Gondwana continued to split apart, with Africa, South America, Antarctica and Australia pulling away from each other. Africa was heading north toward Europe, slowly closing the Tethys Ocean, and India began its migration to Asia that would lead to a tectonic collision and the formation of the Himalayas.
Is there an answer to this question: In the Paleocene period Greenland was joined to which continent?

Answer: North America


Question: It was temporarily under the control of the Tibetan empire and Chinese from 650–680 and then under the control of the Umayyads in 710. The Samanid Empire, 819 to 999, restored Persian control of the region and enlarged the cities of Samarkand and Bukhara (both cities are today part of Uzbekistan) which became the cultural centers of Iran and the region was known as Khorasan. The Kara-Khanid Khanate conquered Transoxania (which corresponds approximately with modern-day Uzbekistan, Tajikistan, southern Kyrgyzstan and southwest Kazakhstan) and ruled between 999–1211. Their arrival in Transoxania signaled a definitive shift from Iranian to Turkic predominance in Central Asia, but gradually the Kara-khanids became assimilated into the Perso-Arab Muslim culture of the region.
Is there an answer to this question: Who restored Persian control of the region?

Answer:
The Samanid Empire,