Other microscopic procedures may also aid in identifying infectious agents. Almost all cells readily stain with a number of basic dyes due to the electrostatic attraction between negatively charged cellular molecules and the positive charge on the dye. A cell is normally transparent under a microscope, and using a stain increases the contrast of a cell with its background. Staining a cell with a dye such as Giemsa stain or crystal violet allows a microscopist to describe its size, shape, internal and external components and its associations with other cells. The response of bacteria to different staining procedures is used in the taxonomic classification of microbes as well. Two methods, the Gram stain and the acid-fast stain, are the standard approaches used to classify bacteria and to diagnosis of disease. The Gram stain identifies the bacterial groups Firmicutes and Actinobacteria, both of which contain many significant human pathogens. The acid-fast staining procedure identifies the Actinobacterial genera Mycobacterium and Nocardia.
How many methods comprise standard approaches used to classify bacteria and diagnose disease?
Two

Napoleon's triumph at Marengo secured his political authority and boosted his popularity back home, but it did not lead to an immediate peace. Bonaparte's brother, Joseph, led the complex negotiations in Lunéville and reported that Austria, emboldened by British support, would not acknowledge the new territory that France had acquired. As negotiations became increasingly fractious, Bonaparte gave orders to his general Moreau to strike Austria once more. Moreau and the French swept through Bavaria and scored an overwhelming victory at Hohenlinden in December 1800. As a result, the Austrians capitulated and signed the Treaty of Lunéville in February 1801. The treaty reaffirmed and expanded earlier French gains at Campo Formio. Britain now remained the only nation that was still at war with France.
Where did the victory for Napoleon that secured his political power and increased his popularity in France occur?
Marengo

The fourth a cappella musical to appear Off-Broadway, In Transit, premiered 5 October 2010 and was produced by Primary Stages with book, music, and lyrics by Kristen Anderson-Lopez, James-Allen Ford, Russ Kaplan, and Sara Wordsworth. Set primarily in the New York City subway system its score features an eclectic mix of musical genres (including jazz, hip hop, Latin, rock, and country). In Transit incorporates vocal beat boxing into its contemporary a cappella arrangements through the use of a subway beat boxer character. Beat boxer and actor Chesney Snow performed this role for the 2010 Primary Stages production. According to the show's website, it is scheduled to reopen for an open-ended commercial run in the Fall of 2011. In 2011 the production received four Lucille Lortel Award nominations including Outstanding Musical, Outer Critics Circle and Drama League nominations, as well as five Drama Desk nominations including Outstanding Musical and won for Outstanding Ensemble Performance.
What a cappella vocal style is implemented in many of In Transit's songs?
beat boxing