The central oscillator generates a self-sustaining rhythm and is driven by two interacting feedback loops that are active at different times of day. The morning loop consists of CCA1 (Circadian and Clock-Associated 1) and LHY (Late Elongated Hypocotyl), which encode closely related MYB transcription factors that regulate circadian rhythms in Arabidopsis, as well as PRR 7 and 9 (Pseudo-Response Regulators.) The evening loop consists of GI (Gigantea) and ELF4, both involved in regulation of flowering time genes. When CCA1 and LHY are overexpressed (under constant light or dark conditions), plants become arrhythmic, and mRNA signals reduce, contributing to a negative feedback loop. Gene expression of CCA1 and LHY oscillates and peaks in the early morning, whereas TOC1 gene expression oscillates and peaks in the early evening. While it was previously hypothesised that these three genes model a negative feedback loop in which over-expressed CCA1 and LHY repress TOC1 and over-expressed TOC1 is a positive regulator of CCA1 and LHY, it was shown in 2012 by Andrew Millar and others that TOC1 in fact serves as a repressor not only of CCA1, LHY, and PRR7 and 9 in the morning loop but also of GI and ELF4 in the evening loop. This finding and further computational modeling of TOC1 gene functions and interactions suggest a reframing of the plant circadian clock as a triple negative-component repressilator model rather than the positive/negative-element feedback loop characterizing the clock in mammals.
What was the previous belief of feedback in the gene loop?
negative feedback loop


Input: Samoa
Britain also sent troops to protect British business enterprise, harbour rights, and consulate office. This was followed by an eight-year civil war, during which each of the three powers supplied arms, training and in some cases combat troops to the warring Samoan parties. The Samoan crisis came to a critical juncture in March 1889 when all three colonial contenders sent warships into Apia harbour, and a larger-scale war seemed imminent. A massive storm on 15 March 1889 damaged or destroyed the warships, ending the military conflict.

When did the crucial climax of the civil war occur?
Output: March 1889


Input: Article: In parliamentary systems fashioned after the Westminster system, the prime minister is the presiding and actual head of government and head of the executive branch. In such systems, the head of state or the head of state's official representative (i.e. the monarch, president, or governor-general) usually holds a largely ceremonial position, although often with reserve powers.

Now answer this question: What kind of role is the head of state in Westminster-based parliamentary governments?

Output: ceremonial


Article: After Richard's death on 6 April 1199 there were two potential claimants to the Angevin throne: John, whose claim rested on being the sole surviving son of Henry II, and young Arthur I of Brittany, who held a claim as the son of John's elder brother Geoffrey. Richard appears to have started to recognise John as his heir presumptive in the final years before his death, but the matter was not clear-cut and medieval law gave little guidance as to how the competing claims should be decided. With Norman law favouring John as the only surviving son of Henry II and Angevin law favouring Arthur as the only son of Henry's elder son, the matter rapidly became an open conflict. John was supported by the bulk of the English and Norman nobility and was crowned at Westminster, backed by his mother, Eleanor. Arthur was supported by the majority of the Breton, Maine and Anjou nobles and received the support of Philip II, who remained committed to breaking up the Angevin territories on the continent. With Arthur's army pressing up the Loire valley towards Angers and Philip's forces moving down the valley towards Tours, John's continental empire was in danger of being cut in two.

Question: Where was John crowned?
Ans: Westminster


Here is a question about this article: Architecture has to do with planning and designing form, space and ambience to reflect functional, technical, social, environmental and aesthetic considerations. It requires the creative manipulation and coordination of materials and technology, and of light and shadow. Often, conflicting requirements must be resolved. The practice of Architecture also encompasses the pragmatic aspects of realizing buildings and structures, including scheduling, cost estimation and construction administration. Documentation produced by architects, typically drawings, plans and technical specifications, defines the structure and/or behavior of a building or other kind of system that is to be or has been constructed.
What is the answer to this question: Aside from planning and design what other types of aspects does architecture involve?
****
So... pragmatic aspects


Article: An additional 4.6 billion tonnes of uranium are estimated to be in sea water (Japanese scientists in the 1980s showed that extraction of uranium from sea water using ion exchangers was technically feasible). There have been experiments to extract uranium from sea water, but the yield has been low due to the carbonate present in the water. In 2012, ORNL researchers announced the successful development of a new absorbent material dubbed HiCap which performs surface retention of solid or gas molecules, atoms or ions and also effectively removes toxic metals from water, according to results verified by researchers at Pacific Northwest National Laboratory.

Question: Where were ORNL's results verified?
Ans:
Pacific Northwest National Laboratory