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As with bacterial classification, identification of bacteria is increasingly using molecular methods. Diagnostics using DNA-based tools, such as polymerase chain reaction, are increasingly popular due to their specificity and speed, compared to culture-based methods. These methods also allow the detection and identification of "viable but nonculturable" cells that are metabolically active but non-dividing. However, even using these improved methods, the total number of bacterial species is not known and cannot even be estimated with any certainty. Following present classification, there are a little less than 9,300 known species of prokaryotes, which includes bacteria and archaea; but attempts to estimate the true number of bacterial diversity have ranged from 107 to 109 total species – and even these diverse estimates may be off by many orders of magnitude.

What else can molecular methods of classification reveal?
Answer: identification of "viable but nonculturable" cells
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On February 6, 2016, one day before her performance at the Super Bowl, Beyoncé released a new single exclusively on music streaming service Tidal called "Formation".

Beyonce's new single released before the super bowl was called what?
Answer: Formation
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Evidence of Han-era mechanical engineering comes largely from the choice observational writings of sometimes disinterested Confucian scholars. Professional artisan-engineers (jiang 匠) did not leave behind detailed records of their work. Han scholars, who often had little or no expertise in mechanical engineering, sometimes provided insufficient information on the various technologies they described. Nevertheless, some Han literary sources provide crucial information. For example, in 15 BC the philosopher Yang Xiong described the invention of the belt drive for a quilling machine, which was of great importance to early textile manufacturing. The inventions of the artisan-engineer Ding Huan (丁緩) are mentioned in the Miscellaneous Notes on the Western Capital. Around 180 AD, Ding created a manually operated rotary fan used for air conditioning within palace buildings. Ding also used gimbals as pivotal supports for one of his incense burners and invented the world's first known zoetrope lamp.

What type of invention was used to provide air conditioning for the palace buildings?
Answer:
rotary fan