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Older operating systems such as TOPS-10, along with CP/M, tracked file length only in units of disk blocks and used Control-Z (SUB) to mark the end of the actual text in the file. For this reason, EOF, or end-of-file, was used colloquially and conventionally as a three-letter acronym for Control-Z instead of SUBstitute. The end-of-text code (ETX), also known as Control-C, was inappropriate for a variety of reasons, while using Z as the control code to end a file is analogous to it ending the alphabet and serves as a very convenient mnemonic aid. A historically common and still prevalent convention uses the ETX code convention to interrupt and halt a program via an input data stream, usually from a keyboard.

What does EOF stand for?
end-of-file