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In the autumn of 2009, Glee featured the fictional high school's show choir singing "Somebody to Love" as their second act performance in the episode "The Rhodes Not Taken". The performance was included on the show's Volume 1 soundtrack CD. In June 2010, the choir performed "Another One Bites the Dust" in the episode "Funk". The following week's episode, "Journey to Regionals", features a rival choir performing "Bohemian Rhapsody" in its entirety. The song was featured on the episode's EP. In May 2012, the choir performed "We Are the Champions" in the episode "Nationals", and the song features in The Graduation Album.

What Queen song is featured on Glee's The Graduation Album?
We Are the Champions