Problem: The central oscillator generates a self-sustaining rhythm and is driven by two interacting feedback loops that are active at different times of day. The morning loop consists of CCA1 (Circadian and Clock-Associated 1) and LHY (Late Elongated Hypocotyl), which encode closely related MYB transcription factors that regulate circadian rhythms in Arabidopsis, as well as PRR 7 and 9 (Pseudo-Response Regulators.) The evening loop consists of GI (Gigantea) and ELF4, both involved in regulation of flowering time genes. When CCA1 and LHY are overexpressed (under constant light or dark conditions), plants become arrhythmic, and mRNA signals reduce, contributing to a negative feedback loop. Gene expression of CCA1 and LHY oscillates and peaks in the early morning, whereas TOC1 gene expression oscillates and peaks in the early evening. While it was previously hypothesised that these three genes model a negative feedback loop in which over-expressed CCA1 and LHY repress TOC1 and over-expressed TOC1 is a positive regulator of CCA1 and LHY, it was shown in 2012 by Andrew Millar and others that TOC1 in fact serves as a repressor not only of CCA1, LHY, and PRR7 and 9 in the morning loop but also of GI and ELF4 in the evening loop. This finding and further computational modeling of TOC1 gene functions and interactions suggest a reframing of the plant circadian clock as a triple negative-component repressilator model rather than the positive/negative-element feedback loop characterizing the clock in mammals.
When do GI and LHY function?
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Answer: unanswerable


Problem: The event of the Protestant Reformation and the politics that ensued has been cited as the origins of German identity that arose in response to the spread of a common German language and literature. Early German national culture was developed through literary and religious figures including Martin Luther, Johann Wolfgang von Goethe and Friedrich Schiller. The concept of a German nation was developed by German philosopher Johann Gottfried Herder. The popularity of German identity arose in the aftermath of the French Revolution.
What man is credited with the emergence of the German nation?
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Answer: Johann Gottfried Herder


Problem: Zhejiang, as the heartland of the Jiangnan (Yangtze River Delta), remained the wealthiest area during the Six Dynasties (220 or 222–589), Sui, and Tang. After being incorporated into the Sui dynasty, its economic richness was used for the Sui dynasty's ambitions to expand north and south, particularly into Korea and Vietnam. The plan led the Sui dynasty to restore and expand the network which became the Grand Canal of China. The Canal regularly transported grains and resources from Zhejiang, through its metropolitan center Hangzhou (and its hinterland along both the Zhe River and the shores of Hangzhou Bay), and from Suzhou, and thence to the North China Plain. The débâcle of the Korean war led to Sui's overthrow by the Tang, who then presided over a centuries-long golden age for the country. Zhejiang was an important economic center of the empire's Jiangnan East Circuit and was considered particularly prosperous. Throughout the Tang dynasty, The Grand Canal had remained effective, transporting grains and material resources to North China plain and metropolitan centers of the empire. As the Tang Dynasty disintegrated, Zhejiang constituted most of the territory of the regional kingdom of Wuyue.
 What ocean was one of the Grand Canal of China's hinterlands?
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Answer:
unanswerable