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In addition to the use of the multiprotein complexes listed above, Gram-negative bacteria possess another method for release of material: the formation of outer membrane vesicles. Portions of the outer membrane pinch off, forming spherical structures made of a lipid bilayer enclosing periplasmic materials. Vesicles from a number of bacterial species have been found to contain virulence factors, some have immunomodulatory effects, and some can directly adhere to and intoxicate host cells. While release of vesicles has been demonstrated as a general response to stress conditions, the process of loading cargo proteins seems to be selective.

What other method does Gram-negative bacters use to release material?
Answer: the formation of outer membrane vesicles
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In some decorative applications, wood with knots may be desirable to add visual interest. In applications where wood is painted, such as skirting boards, fascia boards, door frames and furniture, resins present in the timber may continue to 'bleed' through to the surface of a knot for months or even years after manufacture and show as a yellow or brownish stain. A knot primer paint or solution, correctly applied during preparation, may do much to reduce this problem but it is difficult to control completely, especially when using mass-produced kiln-dried timber stocks.

What term is used to describe what knots can do to affect the finish of things made from wood even when they've been painted?
Answer: bleed
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It is in this time that the notation of music on a staff and other elements of musical notation began to take shape. This invention made possible the separation of the composition of a piece of music from its transmission; without written music, transmission was oral, and subject to change every time it was transmitted. With a musical score, a work of music could be performed without the composer's presence. The invention of the movable-type printing press in the 15th century had far-reaching consequences on the preservation and transmission of music.

When was the movable-type printing press invented?
Answer:
the 15th century