The hagiography of Mary and the Holy Family can be contrasted with other material in the Gospels. These references include an incident which can be interpreted as Jesus rejecting his family in the New Testament: "And his mother and his brothers arrived, and standing outside, they sent in a message asking for him ... And looking at those who sat in a circle around him, Jesus said, 'These are my mother and my brothers. Whoever does the will of God is my brother, and sister, and mother'."[3:31-35] Other verses suggest a conflict between Jesus and his family, including an attempt to have Jesus restrained because "he is out of his mind", and the famous quote: "A prophet is not without honor except in his own town, among his relatives and in his own home." A leading Biblical scholar commented: "there are clear signs not only that Jesus's family rejected his message during his public ministry but that he in turn spurned them publicly".

Who did Jesus say were his "brother, and sister, and mother?"