Circadian rhythm
The earliest recorded account of a circadian process dates from the 4th century B.C.E., when Androsthenes, a ship captain serving under Alexander the Great, described diurnal leaf movements of the tamarind tree. The observation of a circadian or diurnal process in humans is mentioned in Chinese medical texts dated to around the 13th century, including the Noon and Midnight Manual and the Mnemonic Rhyme to Aid in the Selection of Acu-points According to the Diurnal Cycle, the Day of the Month and the Season of the Year.

Q: What is the earliest recorded instance of circadian rhythm?
4th century B.C.E