But Bt cotton is ineffective against many cotton pests, however, such as plant bugs, stink bugs, and aphids; depending on circumstances it may still be desirable to use insecticides against these. A 2006 study done by Cornell researchers, the Center for Chinese Agricultural Policy and the Chinese Academy of Science on Bt cotton farming in China found that after seven years these secondary pests that were normally controlled by pesticide had increased, necessitating the use of pesticides at similar levels to non-Bt cotton and causing less profit for farmers because of the extra expense of GM seeds. However, a 2009 study by the Chinese Academy of Sciences, Stanford University and Rutgers University refuted this. They concluded that the GM cotton effectively controlled bollworm. The secondary pests were mostly miridae (plant bugs) whose increase was related to local temperature and rainfall and only continued to increase in half the villages studied. Moreover, the increase in insecticide use for the control of these secondary insects was far smaller than the reduction in total insecticide use due to Bt cotton adoption. A 2012 Chinese study concluded that Bt cotton halved the use of pesticides and doubled the level of ladybirds, lacewings and spiders. The International Service for the Acquisition of Agri-biotech Applications (ISAAA) said that, worldwide, GM cotton was planted on an area of 25 million hectares in 2011. This was 69% of the worldwide total area planted in cotton.

In what year did a research study find that the use of Bt toxin plants failed to be effective after 7 years?