Context and question: Microbiological culture is a principal tool used to diagnose infectious disease. In a microbial culture, a growth medium is provided for a specific agent. A sample taken from potentially diseased tissue or fluid is then tested for the presence of an infectious agent able to grow within that medium. Most pathogenic bacteria are easily grown on nutrient agar, a form of solid medium that supplies carbohydrates and proteins necessary for growth of a bacterium, along with copious amounts of water. A single bacterium will grow into a visible mound on the surface of the plate called a colony, which may be separated from other colonies or melded together into a "lawn". The size, color, shape and form of a colony is characteristic of the bacterial species, its specific genetic makeup (its strain), and the environment that supports its growth. Other ingredients are often added to the plate to aid in identification. Plates may contain substances that permit the growth of some bacteria and not others, or that change color in response to certain bacteria and not others. Bacteriological plates such as these are commonly used in the clinical identification of infectious bacterium. Microbial culture may also be used in the identification of viruses: the medium in this case being cells grown in culture that the virus can infect, and then alter or kill. In the case of viral identification, a region of dead cells results from viral growth, and is called a "plaque". Eukaryotic parasites may also be grown in culture as a means of identifying a particular agent.
What type of medium is not typically provided for a specific agent in a microbial culture?
Answer: unanswerable
Context and question: De novo or "orphan" genes, whose sequence shows no similarity to existing genes, are extremely rare. Estimates of the number of de novo genes in the human genome range from 18 to 60. Such genes are typically shorter and simpler in structure than most eukaryotic genes, with few if any introns. Two primary sources of orphan protein-coding genes are gene duplication followed by extremely rapid sequence change, such that the original relationship is undetectable by sequence comparisons, and formation through mutation of "cryptic" transcription start sites that introduce a new open reading frame in a region of the genome that did not previously code for a protein.
What is the estimate of the number of orphan genes in the human genome?
Answer: 18 to 60
Context and question: In the middle of the 17th century, the peasant rebel leader Zhang Xianzhong (1606–1646) from Yan'an, Shanxi Province, nicknamed Yellow Tiger, led his peasant troop from north China to the south, and conquered Sichuan. Upon capturing it, he declared himself emperor of the Daxi Dynasty (大西王朝). In response to the resistance from local elites, he massacred a large native population. As a result of the massacre as well as years of turmoil during the Ming-Qing transition, the population of Sichuan fell sharply, requiring a massive resettlement of people from the neighboring Huguang Province (modern Hubei and Hunan) and other provinces during the Qing dynasty.
Which province supplied a large amount of troops to the Sichuan resettlement, following years of turmoil during the Ming-Qing transition?
Answer:
unanswerable